Daily Archives: April 20, 2015

Incompetence, Mismanagement Plague California’s Obamacare Insurance Exchange

“Now, several senior-level officials integral to the launch of Covered California—who enthusiastically support the Affordable Care Act—are speaking about what they view as gross incompetence and mismanagement involving some of the $1 billion federal tax dollars poured into the state effort.” click here

For more on the Obamacare disaster click here.

Assumed “Age of the Earth” Confounds Climate Study

These types of studies rely a circular reasoning resulting in a assumed age of the earth. The geologic strata examined in this study may or may not say anything about the “age of the earth”, glaciation in North America, or CO2. The conclusions drawn here are not much more than a guess, albeit an educated guess, but a guess nevertheless.

“approximately four million years ago large climatic changes started to emerge that promoted increased glaciation of North America. Since one million years ago this effect has even been amplified in response to the drastic alternations between warm and cold phases of the ice ages.” here for more

Fluoride Effect on Human Cells

Like other studies of its kind, this abstract begins with a dogmatic statement for which no evidence is provided.

Wang H, Jiao Y, Ma C, Zhang H, Zhou Z. Effect of Sodium Fluoride on the Proliferation and Gene Differential Expression in Human RPMI8226 Cells. Biological Trace Element Research 2015 Mar 1.

Although fluoride is known to reduce the incidence of caries, chronic excessive fluoride exposure can impair human health, even resulting in fluorosis. Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating the effect of sodium fluoride (NaF) in human RPMI8226 cells. In this experiment, human RPMI8226 cells were cultured with varied doses of fluoride (10, 20, 40, 80, 160, 320 μM). After 48 h exposure, the change of cell viability was examined by CCK-8 assay, and also the messenger RNA (mRNA) expression of relevant genes was assessed by QRT-PCR. Compared to the control group, fluoride exposure increased the human RPMI8226 cells viability at relatively lower levels (10-160 μM); however, when the concentration reached to 320 μM, the cell proliferation was significantly inhibited (p < 0.05). In addition, the genes mRNA expression, including ANKRD1, CRSP6, KLF2, SBNO2, ZNF649, FANCM, PDGFA, RNF152, CDK10, and CETN2 changed in a concentration-dependent manner and increased with fluoride exposure concentration. The results suggest that overexposure to fluoride (160-320 μM) can induce cytotoxicity and regulate relevant genes expression. Our findings provide novel insights into the mechanisms of action of fluoride-induced toxicity.

Paper is here (Open Access).