Daily Archives: June 23, 2015

EPA “Global Action” Climate Report Contains Nonsense Statements

The EPA Global Action report contains unsubstantiated statements that are pure speculation at best. But some claims are simply nonsense such as global action will cause an “estimated 40%-59% fewer severe and extreme droughts” in the U.S. by the year 2100. Emission reductions will have none to very little if any affect on severe and extreme drought in the Southwest. 

High Performance Concentration Method for Viruses in Drinking Water

Kunze A, Pei L, Elsässer D, Niessner R, Seidel M. High performance concentration method for viruses in drinking water. Journal of Virological Methods. 2015 Jun 18. pii: S0166-0934(15)00223-2. doi: 10.1016/j.jviromet.2015.06.007.

According to the risk assessment of the WHO, highly infectious pathogenic viruses like rotaviruses should not be present in large-volume drinking water samples of up to 90m3. On the other hand, quantification methods for viruses are only operable in small volumes, and presently no concentration procedure for processing such large volumes has been reported. Therefore, the aim of this study was to demonstrate a procedure for processing viruses in-line of a drinking water pipeline by ultrafiltration (UF) and consecutive further concentration by monolithic filtration (MF) and centrifugal ultrafiltration (CeUF) of viruses to a final 1-mL sample. For testing this concept, the model virus bacteriophage MS2 was spiked continuously in UF instrumentation. Tap water was processed in volumes between 32.4m3 (22h) and 97.7m3 (72h) continuously either in dead-end (DE) or cross-flow (CF) mode. Best results were found by DE-UF over 22h. The concentration of MS2 was increased from 4.2×104GU/mL (genomic units per milliliter) to 3.2×1010GU/mL and from 71PFU/mL to 2×108PFU/mL as determined by qRT-PCR and plaque assay, respectively.