Daily Archives: July 17, 2015

Detoxification of Cr6 to Cr3 in the Stomach

This is an important study for chromium 6 risk assessment. Humans exhibit a relatively high capacity for Cr6 reduction to Cr3 in the stomach prior to intestinal absorption. This is an important detoxification mechanism for Cr6.

Sasso AF, Schlosser PM. An evaluation of in vivo models for toxicokinetics of hexavalent chromium in the stomach. Toxicology and applied pharmacology. 2015 Jun 27. pii: S0041-008X(15)30023-5. doi: 10.1016/j.taap.2015.06.016.

Hexavalent chromium (Cr6) is a drinking water contaminant that has been detected in most of the water systems throughout the United States. In 2-year drinking water bioassays, the National Toxicology Program (NTP) found clear evidence of carcinogenic activity in male and female rats and mice. Because reduction of Cr6 to trivalent chromium (Cr3) is an important detoxifying step in the gastrointestinal (GI) tract prior to systemic absorption, models have been developed to estimate the extent of reduction in humans and animals. The objective of this work was to use a revised model of ex vivo Cr6 reduction kinetics in gastric juice to analyze the potential reduction kinetics under in vivo conditions for mice, rats and humans. A published physiologically-based pharmacokinetic (PBPK) model was adapted to incorporate the new reduction model. This paper focuses on the toxicokinetics of Cr6 in the stomach compartment, where most of the extracellular Cr6 reduction is believed to occur in humans. Within the range of doses administered by the NTP bioassays, neither the original nor revised models predict saturation of stomach reducing capacity to occur in vivo if applying default parameters. However, both models still indicate that mice exhibit the lowest extent of reduction in the stomach, meaning that a higher percentage of the Cr6 dose may escape stomach reduction in that species. Similarly, both models predict that humans exhibit the highest extent of reduction at low doses.

Jindal: “Radical Islam is at War with Us”

“Louisiana Gov. Bobby Jindal, a 2016 GOP presidential candidate, is reacting to the shooting in Chattanooga, Tennessee, where four U.S. marines were killed, allegedly by Kuwaiti-born 24-year-old Muhammad Youssef Abdulazeez.”
Full article is here.

Water Treatment Affecting Bacterial Antibiotic Resistance

Bai X, Ma X, Xu F, Li J, Zhang H, Xiao X. The drinking water treatment process as a potential source of affecting the bacterial antibiotic resistance. Sci Total Environ. 2015 Jul 3;533:24-31. doi: 10.1016/j.scitotenv.2015.06.082.

Two waterworks, with source water derived from the Huangpu or Yangtze River in Shanghai, were investigated, and the effluents were plate-screened for antibiotic-resistant bacteria (ARB) using five antibiotics: ampicillin (AMP), kanamycin (KAN), rifampicin (RFP), chloramphenicol (CM) and streptomycin (STR). The influence of water treatment procedures on the bacterial antibiotic resistance rate and the changes that bacteria underwent when exposed to the five antibiotics at concentration levels ranging from 1 to 100 μg/mL were studied. Multi-drug resistance was also analyzed using drug sensitivity tests. The results indicated that bacteria derived from water treatment plant effluent that used the Huangpu River rather than the Yangtze River as source water exhibited higher antibiotic resistance rates against AMP, STR, RFP and CM but lower antibiotic resistance rates against KAN. When the antibiotic concentration levels ranged from 1 to 10 μg/mL, the antibiotic resistance rates of the bacteria in the water increased as water treatment progressed. Biological activated carbon (BAC) filtration played a key role in increasing the antibiotic resistance rate of bacteria. Chloramine disinfection can enhance antibiotic resistance. Among the isolated ARB, 75% were resistant to multiple antibiotics. Ozone oxidation, BAC filtration and chloramine disinfection can greatly affect the relative abundance of bacteria in the community.